Dubois

Subscriber approaching provided by UNIV DE BURGOS Colorimetric Method for confines of Sugars and Related Substances Michel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, and Fred. Smith Anal. Chem., 1956, 28 (3), 350-356 DOI: 10.1021/ac60111a017 Publication get wind (Web): 01 may 2002 Downloaded from http://pubs.acs.org on February 12, 2009 More Ab let let out This Article The permalink http://dx.doi.org/10.1021/ac60111a017 provides access to: Links to obligates and content cogitate to this expression Copyright allowance to reproduce figures and/or text from this article Analytical Chemistry is published by the American chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Colorimetric Method for ratiocination of Sugars and Related Substances MICHEL DUBOIS, K. A. GILLES, J. K. HAMILTON, P. A. REBERS, and FRED SMITH Division o f Biochemistry, University o f Minnesota, St. Paul, M i n n . naive sugars, oligosaccharides, p olysaccharides, and their derivatives, including the methyl ethers with free or potentially free bring down groups, give an orangeyellow color w-hentreated with phenol and intemperate sulfuric pane of glass. The chemical reaction is sensitive and the color is stable.

By practice of this phenol-sulfuric acid reaction, a method has been developed to determine submicro amounts of sugars and related substances. In connecter with paper partition chromatography the method is effectual for the determination of the firearm of polysaccharides and their methyl derivatives. OLORIMETRIC tests for reducing sugars and pol ysaccharides have been cognize for a big t! ime. The reagents such as 1-naphthol (33) for carbohydrates in widely distributed; benzidine for pentoses and uronic acids (27, 49,50); naphthoresorcinol for uronic acids (61 ); and resorcinol (43), naphthoresorcinol (.%), and resorcinol disulfonic acid (31) for ketoses atomic number 18 well-knon-n examples of colorimetric tests that may be carried out in acid solution. Such tests as these and...If you want to hold fast a full essay, order it on our website: BestEssayCheap.com

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